Electrophoresis of Human Serum Albumin at pH 4.0
نویسندگان
چکیده
منابع مشابه
Electrophoretic Behavior of Normal Human Serum Albumin at pH 4.0
In the preceding paper on the behavior of pooled normal human serum albumin in various buffers at pH 4.0 (1)) it was demonstrated that the electrophoretic pattern of albumin shows a single moving boundary in the presence of the lower fatty acids, excepting formic and acetic acid, and of certain diand tribasic organic acids. This phenomenon is probably a consequence of the interaction between th...
متن کاملElectrophoresis of human serum albumin at pH 4.0. I. A systematic study on the effect of organic acids and alcohols upon the electrophoretic behavior of albumin.
Recently we have reported on a hitherto unknown modification of human serum albumin of which the outstanding properties are electrophoretic homogeneity at pH 4.0 and its transformation to an electrophoretically heterogeneous form (2). In continuation of this study, the question was asked whether the ordinary human serum albumin (Fraction V), known to bc electrophoretically heterogeneous at pH 4...
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A 51-year old female patient with newly diagnosed chronic kidney disease (CKD) presented to the hospital with an acute exacerbation of chronic obstructive pulmonary disease (COPD). On admission her serum creatinine was 247 μmol/L and urea was 15 mmol/L. The patient was a known hypertensive and was on irregular treatment. The CKD was probably a result of hypertensive nephropathy. As the liver fu...
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Human serum albumin (HSA), under conditions of low pH, is known to exist in two isomeric forms, the F form at around pH 4.0 and the E form below 3.0. We studied its conformation in the acid-denatured E form using far-UV and near-UV CD, binding of a hydrophobic probe, 1-anilinonaphthalene-8-sulfonic acid (ANS), thermal transition by far-UV and near-UV CD, tryptophan fluorescence, quenching of tr...
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Background and Aims: Some nanoparticles can be used in immunoassays to increase sensitivity. This study aimed to evaluate a novel nano-immunoassay based on bovine serum albumin nanoparticles (BSA NPs). Materials and methods: At first, the nanostructure was synthesized, and then applied as a tag in the nano-immunoassay. Then the concentration of β-subunit of human chorionic gonadotropin ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1959
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)69641-3